Amino acids present in protein formulations serve as buffers, bulking agents, stabilizers, and antioxidants. Eight specific amino acids are commonly used as excipients in protein therapeutic formulations due to their physicochemical properties. These include Arginine, Aspartic acid, Glutamic acid, Lysine, Proline, Glycine, Histidine, and Methionine. The UHPLC column, an Acclaim PA2 column, provides excellent separation characteristics for these un-labeled amino acids. Gradient conditions are adjusted by selecting appropriate ion-pairing reagents, pH and level of organic solvents.

The use of Thermo Scientific Accucore C30 HPLC columns for the characterization of different cooking oils is demonstrated. The C30 phase offers greater selectivity and resolution of minor components when compared to the C18 phase.

This work demonstrates the determination of the API (amphetamine) and counterions (aspartate, saccharin and sulfate) in a medicine Adderall within a single analysis using a Thermo Scientific Acclaim Trinity P2 column and Thermo Scientific Dionex Corona Veo Charged Aerosol Detector (CAD).

The Thermo Scientific Dionex UltiMate 3000 HPLC system is applied for the analysis of polyacrylic acid in boiler water. The separation is performed on a Thermo Scientific Acclaim SEC-1000 column, with charged aerosol detection.

The Thermo Scientific Dionex UltiMate 3000 RS system is applied for the analysis of electrolytes in sports beverages. The separation is performed on a Thermo Scientific Acclaim Trinity P2 column, which is designed to resolve a broad range of anions and cations, mono- or multi-valent, in a single analysis using a simple gradient method. The Thermo Scientific OnGuard II P cartridge was used to remove artificial colors from the samples.