Metformin (N,N-dimethylimidodicarbonimidic diamide) is a widely prescribed orally administered antidiabetic drug for the treatment of type 2 diabetes. This application presents a IC method used for quantitation of dimethylamine in metformin hydrochloride. This method uses a Thermo Scientific Dionex IonPac CS19 cation-exchange analytical column with methanesulfonic acid eluent produced by an eluent generator. The method separates dimethylamine from other cations typically present in drug products.

The Thermo Scientific HPLC system is applied for the separation of basic compounds. The separation was performed using a Thermo Scientific Accucore RP-MS column in under 3 minutes, with UV detection at 215nm.

A Thermo Scientific HPLC system is applied with the Thermo Scientific Accucore 2.6 µm C18 column for the analysis of a range of benzoic acids in less than one minute with UV detection at 254 nm.

The Thermo Scientific Acclaim Mixed-Mode HILIC-1 column is used for the analysis of cytosine and naphthalene in both reversed-phase and HILIC mode, and the retention compared.

The Thermo Scientific HPLC system is applied for the analysis of acids, bases and neutrals. The separation is performed on a Thermo Scientific Hypersil GOLD HPLC column, with UV detection at 270 nm.