To describe an accurate and precise high-throughput analytical technique for the analysis of methylmalonic acid (MMA) utilizing Thermo Scientific SOLAµ WAX followed by LC-MS/MS. This technique further separates MMA from the known endogenous interference succinic acid (SA), a compound that has the same molecular weight as MMA and demonstrates a similar fragmentation pattern, which can cause issues with selectivity in mass spectrometry (MS) detection. For Research Use Only. Not for use in diagnostic procedures.

The separation of perfluoro-octanoic acid (PFOA) and perfluorooctanesulfonic acid (PFOS) in tap water was performed on a Thermo Scientific Acclaim PolarAdvantage II (PA2) HPLC column, with on-line sample concentration and suppressed conductivity detection. Chromatogram A is Sunnyvale tap water spiked with PFOA and PFOS and chromatogram B is a control.

The Thermo Scientific HPLC system is applied for the separation of naphthenic acid. The separation is performed on a Thermo Scientific Acclaim 120 C18 3 μm HPLC column, with UV detection at 210 nm. Naphthenic acid is produced by oxidation of the naphtha fraction of petroleum distillate, and contains hundreds of components. The Thermo Scientific Acclaim 120 C18 3 μm 3 × 250 mm column provides extremely high plate counts per column and thus superior peak capacity for highly complex samples such as this.

The Thermo Scientific HPLC system is applied for the analysis of tartaric and quinic acids. The separation achieved on a Thermo Scientific Acclaim Mixed-Mode WAX-1 column is compared to the separation achieved on a Thermo Scientific Acclaim Organic Acid column. The unique chemistry of the Thermo Scientific Acclaim Mixed-Mode WAX-1 column cleanly separates these compounds based on different ionizations.

The Thermo Scientific HPLC system is applied for the analysis of lisinopril. The separation is performed on a Thermo Scientific Accucore RP-MS column, with UV detection at 210 nm.