The Thermo Scientific Dionex UltiMate 3000 LC system is applied to the separation of related impurities and Intermediate stage separation of Guanine. The separation was performed on a Thermo Scientific Acclaim Mixed Mode WCX-1 column with a UV detection at 254 nm. With a run time of 15 minutes, the method allows easy separation of intermediate stages and related impurities of Guanine.

The Thermo Scientific Acclaim Mixed-Mode WCX-1 column is used for the fast analysis of minoxidil in formulation with a run time of less than 3 minutes.

Patulin and hydroxymethylfurfural (HMF) are both weakly hydrophobic, neutral compounds, and co-elute on many reversed-phase columns. Fruit juice is a complex matrix, so some form of sample cleanup is necessary. In this work, in-line solid phase extraction is performed. The Thermo Scientific Acclaim Mixed-Mode WCX-1 permits organic acids and phenolics to wash out by ion exclusion well ahead of the HMF and patulin (which co-elute on this column). The auxiliary valve transfers the analytes to the Acclaim C30 column that provides excellent resolution of these two substances.

In this example, pork was extracted with organic solvents in a Thermo Scientific ASE Accelerated Solvent Extractor. Evaporation and reconstitution were difficult, so it was overcame by using solid-phase extraction (SPE). SPE is most effective when it is orthogonal to the analytical separation, so a Thermo Scientific Acclaim Mixed-Mode WCX-1 cartridge was used to retain the drugs, and washed away lipids and other interferences with organic solvent. The auxiliary valve then transferred the drugs to an Acclaim C30 column for RP-HPLC analysis.

The Thermo Scientific UltiMate 3000 HPLC system is applied for the analysis of melamine in liquid milk and milk powder. The separation is performed on a Thermo Scientific Acclaim Mixed-Mode WCX-1 HPLC column, with UV detection at 240 nm. The Thermo Scientific Acclaim Mixed-Mode WCX-1 column features a mixed-mode silica-based packing material that incorporates both hydrophobic and weak cation-exchange properties, and demonstrates great potential for separating samples that contain a mixture of ionic and neutral compounds.