Concentration of patulin and hydroxymethylfurfural in apple juice using in-line SPE
DescriptionPatulin and hydroxymethylfurfural (HMF) are both weakly hydrophobic, neutral compounds, and co-elute on many reversed-phase columns. Fruit juice is a complex matrix, so some form of sample cleanup is necessary. In this work, in-line solid phase extraction is performed. The Thermo Scientific Acclaim Mixed-Mode WCX-1 permits organic acids and phenolics to wash out by ion exclusion well ahead of the HMF and patulin (which co-elute on this column). The auxiliary valve transfers the analytes to the Acclaim C30 column that provides excellent resolution of these two substances.
|Market:||Food and Beverage|
|Keywords:||Acclaim C30, Acclaim Mixed-Mode WCX-1, Apple juice, HPLC, hydroxymethylfurfural, Patulin, SPE, UV detection|
For Research Use Only. Not for use in diagnostic procedures.