Concentration of patulin and hydroxymethylfurfural in apple juice using in-line SPE

Instrument Type: HPLC

Patulin and hydroxymethylfurfural (HMF) are both weakly hydrophobic, neutral compounds, and co-elute on many reversed-phase columns. Fruit juice is a complex matrix, so some form of sample cleanup is necessary. In this work, in-line solid phase extraction is performed. The Thermo Scientific Acclaim Mixed-Mode WCX-1 permits organic acids and phenolics to wash out by ion exclusion well ahead of the HMF and patulin (which co-elute on this column). The auxiliary valve transfers the analytes to the Acclaim C30 column that provides excellent resolution of these two substances.