In this Application Update, we combine into a single method individual improvements made to the standard AAA-Direct gradient program. These individual improvements are now in the AAA-Direct manual. This update shows that such combined changes to the AAA-Direct method improve the performance of our previously published method. This new method successfully determines amino acids in yeast extract-peptone-dextrose yeast culture medium (YPD Broth), and Dulbecco’s modified Eagle’s (with F-12), M199, L-15 (Leibovitz), and McCoy’s 5A media for mammalian cell culture.
This work demonstrates the determination of the API (amphetamine) and counterions (aspartate, saccharin and sulfate) in a medicine Adderall within a single analysis using a Thermo Scientific Acclaim Trinity P2 column and Thermo Scientific Dionex Corona Veo Charged Aerosol Detector (CAD).
Therapeutic proteins (antibodies and vaccines) vary considerably due to the nature and dose of the protein molecule. Aggregation is a major degradation pathway of protein therapeutics during storage. Stabilization of these protein formulations can be enhanced through the addition of specific excipients such as surfactants, amino acids and sugars. The separation of therapeutic protein and amino acid excipients was performed using a 2D approach. An integrated UHPLC system with a UV and universal charged aerosol detection offering multi-mode detection for the simultaneous analysis of both non-chr
The Thermo Scientific UltiMate 3000 HPLC system is applied for the analysis of the API (active pharmaceutical ingredient) amphetamine and its counterions. The separation is performed using a Thermo Scientific Acclaim Trinity P1 column, with both UV and charged aerosol detection. Amphetamine and saccharin respond to UV detection; aspartate, saccharin and sulfate respond to charged-aerosol detection. Note that metal contamination will cause peak tailing, and before use, the column should be cleaned with a chelating agent at pH 4.
The Thermo Scientific UltiMate 3000 HPLC system is applied for the analysis of calcium and magnesium in a dietary mineral supplement. The separation is performed using a Thermo Scientific Acclaim Trinity P2 column, with charged aerosol detection. The Thermo Scientific Acclaim Trinity P2 column offers ideal selectivity for various cations and anions including organic acids. In this case, all label claimed minerals and their counterions are confirmed.
