Optimization and validation of a multi-residue LC-MS/MS method for pesticides in spinach and cabbage by using Thermo Scientific Vanquish UHPLC and TSQ Quantis mass spectrometer. Sample preparation was performed using QuEChERS method that provided high recoveries for an extensive scope of pesticides in several simple steps. Data acquisition and processing were carried out using Thermo Scientific TraceFinder software. The optimized method was validated as per the SANTE guideline and applied to real samples demonstrating that the workflow is fit for purpose.
Multi-residue instrumental method that can be applied for high-throughput quantitation of pesticide residues in garlic and cumin at or below the current legislative requirements. The Thermo Scientific Orbitrap Exploris 120 mass spectrometer was operated in two different workflows: using full-scan Data-Independent Acquisition for quantitation and confirmation and using Thermo Scientific AcquireX intelligent data acquisition background exclusion workflow for full spectrum filtering, retrospective analysis, and multi-parameter-based compound identification.
As agricultural trade grows and food safety concerns mount, stricter pesticide regulations are being enforced around the world. Increased pesticide testing and reductions in maximum permissible residue levels have driven demand for fast, sensitive and cost-effective analytical methods for high-throughput screening of multi-class pesticides in food. Detection of 510 pesticides at low ppb levels was achieved within 12 minutes using the Thermo Scientific Exactive benchtop LC/MS system powered by Orbitrap technology.
We describe a method to analyze large numbers of pesticides in a single run on a triple quadrupole mass spectrometer using TraceFinder software with built-in workflows for streamlining method development and routine analysis.
We present a multi-residue analysis method enabled by Thermo Scientific Pesticide Explorer Collection Standard Quantitation Solution. LC–triple-stage MS provides rapid and robust quantitation of more than 250 pesticides below their required MRL. This comprehensive solution includes the QuEChERS sample preparation kit, Dionex UltiMate 3000 LC system, TSQ Endura triple quadrupole MS, TraceFinder software, Accucore aQ column. Method parameters provide a start-to-finish workflow for pesticide analysis. Results address stringent EU guidelines concerning sensitivity, accuracy and precision.
