To demonstrate the application of a software-based method development approach for the advanced separation of all known water-soluble vitamins in reversed-phase mode with ultraviolet detection. To achieve transferability of the predicted method with excellent agreement of predicted and experimental retention times.
To describe a workflow for the simultaneous quantitative analysis of fatsoluble and water-soluble vitamins in drinks and food supplement tablets using the Thermo Scientific™ Vanquish™ Flex Duo UHPLC system and the Thermo Scientific™ Acclaim™ Polar Advantage II column.
The Acclaim PA2 column can successfully analyze a sample with a mobile phase from 100% aqueous to 100% organic solvent, thereby allowing water- and fat-soluble vitamins to be separated in a single analysis. The Dionex UltiMate 3000 system and Chromeleon software facilitate this analysis by allowing multiple injections during the same run. This method is judged accurate, based on analysis of multivitamin dry syrup and a spiked placebo product. The Acclaim PA2 column, combined with an UltiMate 3000 system, is an excellent solution for vitamin determinations.
Many fast UHPLC and traditional HPLC methods suffer under delays caused by the need to wash and equilibrate the column after the separation. Typically we see 20%–40% of the runtime used up by these essential steps within a gradient method. Here a solution utilizing a dual-gradient pump setup with column switching capabilities to overcome these delays without the need of redeveloping existing methods is offered. The Thermo Scientific™ Dionex™ UltiMate™ 3000 LC Dual System with the Viper Tandem Operation kit allows to sppeding up gradients in LC and UHPLC assays.
We coupled a HRAM instrument to an ultra high pressure LC. The optimized LC method decreased the total analysis time from 130 to 25 minutes. Considerable time and cost savings facilitate routine process monitoring and quality control applications. Reproducible chromatography combined with reliable highly accurate mass measurements on the LTQ Orbitrap enable fast profiling of complex mixtures for their comparison (typing). Also,unknown components of the mixtures can be identified and their chemical structures confidently assigned using the MSn fragmentation capabilities of the LTQ Orbitrap.
