To demonstrate the application of a software-based method development approach for the advanced separation of all known water-soluble vitamins in reversed-phase mode with ultraviolet detection. To achieve transferability of the predicted method with excellent agreement of predicted and experimental retention times.
To describe a workflow for the simultaneous quantitative analysis of fatsoluble and water-soluble vitamins in drinks and food supplement tablets using the Thermo Scientific™ Vanquish™ Flex Duo UHPLC system and the Thermo Scientific™ Acclaim™ Polar Advantage II column.
The Acclaim PA2 column can successfully analyze a sample with a mobile phase from 100% aqueous to 100% organic solvent, thereby allowing water- and fat-soluble vitamins to be separated in a single analysis. The Dionex UltiMate 3000 system and Chromeleon software facilitate this analysis by allowing multiple injections during the same run. This method is judged accurate, based on analysis of multivitamin dry syrup and a spiked placebo product. The Acclaim PA2 column, combined with an UltiMate 3000 system, is an excellent solution for vitamin determinations.
• To demonstrate the capability of the Thermo Scientific™ Acclaim™ PolarAdvantage II (PA2) and Thermo Scientific™ Vanquish™ Flex Binary UHPLC system combination for the rapid separation of water soluble vitamins in sports beverages with excellent linearity, reproducibility, resolution, and recoveries. • To show the capability of the Vanquish Flex Binary UHPLC system to provide superior retention time precision through its novel pump features.
The Thermo Scientific Hypersil GOLD HILIC column was used to separate water soluble vitamins using UV detection at 205, 230 and 260 nm in under 8 minutes.
