Many fast UHPLC and traditional HPLC methods suffer under delays caused by the need to wash and equilibrate the column after the separation. Typically we see 20%–40% of the runtime used up by these essential steps within a gradient method. Here a solution utilizing a dual-gradient pump setup with column switching capabilities to overcome these delays without the need of redeveloping existing methods is offered. The Thermo Scientific™ Dionex™ UltiMate™ 3000 LC Dual System with the Viper Tandem Operation kit allows to sppeding up gradients in LC and UHPLC assays.
The Thermo Scientific HPLC system is applied for the analysis of vitamins and other ingredients in an energy drink. The separation is performed on a Thermo Scientific Acclaim PolarAdvantage (PA) column, with UV detection.
The Thermo Scientific Dionex UltiMate 3000 RSLC is applied for the analysis of water soluble vitamins from soft drink. The separation was performed in less than 5 minutes on a Thermo Scientific Acclaim RSLC PolarAdvantage II (PA2) 2.2 µm column with UV detection.
The Thermo Scientific Dionex UltiMate 3000 RS system is applied for the analysis of seven water-soluble vitamins. The separation was performed in less than 12 minutes on a Thermo Scientific Acclaim C30 5 µm column, with UV detection at 210 nm.
The separation of the ingredients present in an energy drink is performed in less than 16 minutes using a Thermo Scientific Acclaim PA2 3 µm column.
