Optimization and validation of a multi-residue LC-MS/MS method for pesticides in spinach and cabbage by using Thermo Scientific Vanquish UHPLC and TSQ Quantis mass spectrometer. Sample preparation was performed using QuEChERS method that provided high recoveries for an extensive scope of pesticides in several simple steps. Data acquisition and processing were carried out using Thermo Scientific TraceFinder software. The optimized method was validated as per the SANTE guideline and applied to real samples demonstrating that the workflow is fit for purpose.
We present a multi-residue instrumental method that can be applied for high-throughput screening and semi-quantitation of pesticide residues in food matrices at or below the current legislative requirements. A high-resolution, accurate-mass mass spectrometer operated in Full Scan – Variable Data-Independent Analysis (FS-vDIA)* mode provided an option for full spectrum filtering, retrospective analysis, and multi-parameter-based compound identification. The method was validated for 328 target pesticides, with an option for the future extension to a larger number. (*vDIA not available in US.)
As agricultural trade grows and food safety concerns mount, stricter pesticide regulations are being enforced around the world. Increased pesticide testing and reductions in maximum permissible residue levels have driven demand for fast, sensitive and cost-effective analytical methods for high-throughput screening of multi-class pesticides in food. Detection of 510 pesticides at low ppb levels was achieved within 12 minutes using the Thermo Scientific Exactive benchtop LC/MS system powered by Orbitrap technology.
The analysis of food and environmental samples for contaminants by LC-MS has become a quick and cost-effective routine application when run in a targeted fashion, but this method disregards events or circumstances not taken into account beforehand. We describe a method by one data set can serve for routine high throughput quantitative analysis and for versatile non-targeted investigations in a highly automated manner.
We developed a quick and efficient LC-MS/MS method for screening pesticides in orange oil and ginseng extract using TraceFinder software.
