Biomass burning is a major source of particulate matter present in atmospheric aerosol. Anhydrosugars, sugar alcohols, monosaccharides, and disaccharides are among the major classes of water-soluble organic compounds (WSOC) in atmospheric aerosols. Saccharides have been proposed as markers to evaluate the contribution of these sources to atmospheric aerosols. Here, we developed an improved single column HPAE-PAD method to determine anhydrosugars, simple sugars, and sugar alcohols present in atmospheric aerosol samples using CarboPac PA300 column.
We recently introduced a PdH RE for HPAE-PAD carbohydrate determinations. In this work, we demonstrate two popular carbohydrate applications (honey sugars and glycoprotein monosaccharides) using a PdH RE and compare the results to using an Ag/AgCl RE. Our preliminary data indicate that the PdH RE provides comparable results to the standard Ag/AgCl RE for these two applications that use hydroxide eluents.
This application note describes a single column method using HPAE-PAD to determine anhydro sugars, simple sugars, and sugar alcohols present in atmospheric aerosol samples. Using a Dionex CarboPac MA1 column, 11 saccharides can be resolved in a single 55 min method. Additionally, three sugar alcohols can be resolved by modifying the initial eluent concentration,taking the total number of analytes that can be resolved to 14. The 14 saccharides separated include major saccharides found in atmospheric aerosols from four classes—anhydro sugars, sugar alcohols, monosaccharides, and disaccharides.
This study demonstrates use of a commercially available glycopeptide standard as a control to determine glycoprotein hydrolysis reaction efficiency. Because the monosaccharide composition of glycopeptide is known, hydrolysis efficiency under a set of conditions can be easily calculated. The monosaccharide yield for the glycopeptide was compared with that of two glycoproteins under identical conditions. This comparison revealed efficient hydrolysis using the conditions tested here. Minimally the glycopeptide standard serves as a positive control for glycoprotein monosaccharide determination.
The current work describes two methods that use a Dionex CarboPac PA20-4µm column for determination key sugars present in acid hydrolyzed algal biomass. The long method separates the two uronic acids in addition to the twelve other sugars separated by the shorter method. When a detailed sugar composition calculation is desired, the long method can be used. The method was validated for precision, accuracy, and robustness during routine use.
