AB72720: A Glycopeptide Standard for Improving Glycoprotein Hydrolysis Reaction Yield for Accurate Monosaccharide Content Determination
DescriptionThis study demonstrates use of a commercially available glycopeptide standard as a control to determine glycoprotein hydrolysis reaction efficiency. Because the monosaccharide composition of glycopeptide is known, hydrolysis efficiency under a set of conditions can be easily calculated. The monosaccharide yield for the glycopeptide was compared with that of two glycoproteins under identical conditions. This comparison revealed efficient hydrolysis using the conditions tested here. Minimally the glycopeptide standard serves as a positive control for glycoprotein monosaccharide determination.
|Keywords:||Biopharmaceutical, Protein Glycosylation, HPAE-PAD, RFIC System, CarboPac PA20-4μm-Fast column|
|Author:||Sachin Patil and Jeffrey Rohrer|
|Affiliation:||Thermo Fisher Scientific|
For Research Use Only. Not for use in diagnostic procedures.