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AB72720: A Glycopeptide Standard for Improving Glycoprotein Hydrolysis Reaction Yield for Accurate Monosaccharide Content Determination Be the first to rate this application


This study demonstrates use of a commercially available glycopeptide standard as a control to determine glycoprotein hydrolysis reaction efficiency. Because the monosaccharide composition of glycopeptide is known, hydrolysis efficiency under a set of conditions can be easily calculated. The monosaccharide yield for the glycopeptide was compared with that of two glycoproteins under identical conditions. This comparison revealed efficient hydrolysis using the conditions tested here. Minimally the glycopeptide standard serves as a positive control for glycoprotein monosaccharide determination.
Market: BioPharma
Keywords: Biopharmaceutical, Protein Glycosylation, HPAE-PAD, RFIC System, CarboPac PA20-4μm-Fast column, TN 20, TN 70671, TN20, TN70671
Matrix: DI water
Author: Sachin Patil and Jeffrey Rohrer
Affiliation: Thermo Fisher Scientific, Sunnyvale, CA USA
Uploaded on 7/11/2018.

For Research Use Only. Not for use in diagnostic procedures.