This work uses IC with suppressed conductivity and mass spectrometry detection for organic acid determinations. This dual detection approach increases the information available from the sample. Samples were separated with a high-resolution Thermo Scientific™ Dionex™ IonPac™ AS11-HC-4μm column set using a Thermo Scientific™ Dionex™ Integrion™ HPIC™ system with suppressed conductivity detection, and an ISQ EC MS. Co-eluting organic acids can be accurately quantified with mass spectrometric detection, eliminating the need for the addition of methanol to the hydroxide eluent to enhance separation.
Organic acids and their salts such as citric acid, malic acid, formic acid, lactic acid, acetic acid, propionic acid, and fumaric acid are animal feed additives which play an important role in animal. In this application note, the animal feed sample is extracted with water. The extract is filtered or centrifuged and diluted, if necessary. The amounts and types of OAs from the sample are then determined using two IC methods. Method A is based on anion-exchange separation and method B is based on ion-exclusion separation. This is Method A. An ICS-6000 can be used for this application.
The determination of organic acids plays an important fole when testing the authenticity of fruit juices and wines. This application describes a method to determine organic acids in fruit juices and wines using IC with suppressed conductivity detection.
The Thermo Scientific Acclaim Trinity P2 column provides desired selectivity for the separation of mono- and multi-valent anions and cations – a total of twelve ions including sodium, potassium, magnesium, calcium, chloride, bromide, nitrate, malate, citrate, sulfate, fumareate and citrate are well separated on a 50 mm long column using a gradient method within 23 minutes.
Anions and Organic Acids in Brewed Caffeinated Coffee by High Pressure Capillary IC on IonSwift MAX-100 column. This application demonstrates the separation of many organic acids commonly found in complex samples. The method uses the speed advantages of monoliths with the anion-exchange chemistry to separate many organic acids. Additionally, this is a capillary application on ICS-5000 capillary system.
