An AcquireX workflow implemented on the Orbitrap ID-X Tribrid mass spectrometer was evaluated for the analysis of 250 pesticides spiked into strawberry matrix to demonstrate the efficiency of the workflow for identifying the pesticides spiked at different levels. Detection and identification were determined by matching experimentally acquired MS and MS/MS data to validated spectral libraries. The efficiency of the AcquireX workflow was evaluated against a standard data-dependent acquisition and dynamic exclusion method that did not utilize an exclusion list.
Optimization and validation of a multi-residue LC-MS/MS method for pesticides in spinach and cabbage by using Thermo Scientific Vanquish UHPLC and TSQ Quantis mass spectrometer. Sample preparation was performed using QuEChERS method that provided high recoveries for an extensive scope of pesticides in several simple steps. Data acquisition and processing were carried out using Thermo Scientific TraceFinder software. The optimized method was validated as per the SANTE guideline and applied to real samples demonstrating that the workflow is fit for purpose.
We present a multi-residue instrumental method that can be applied for high-throughput screening and semi-quantitation of pesticide residues in food matrices at or below the current legislative requirements. A high-resolution, accurate-mass mass spectrometer operated in Full Scan – Variable Data-Independent Analysis (FS-vDIA)* mode provided an option for full spectrum filtering, retrospective analysis, and multi-parameter-based compound identification. The method was validated for 328 target pesticides, with an option for the future extension to a larger number. (*vDIA not available in US.)
As agricultural trade grows and food safety concerns mount, stricter pesticide regulations are being enforced around the world. Increased pesticide testing and reductions in maximum permissible residue levels have driven demand for fast, sensitive and cost-effective analytical methods for high-throughput screening of multi-class pesticides in food. Detection of 510 pesticides at low ppb levels was achieved within 12 minutes using the Thermo Scientific Exactive benchtop LC/MS system powered by Orbitrap technology.
Red wine is a rich source of phenolic antioxidants and is reported to reduce the risk of diabetes, cancer, Alzheimer's disease, and cardiovascular disease. To improve grape yields it is common practice in vineyards to use pesticides, such as fungicides and insecticides. This application presents a fast, easy, and cost-effective method for the determination of 24 pesticide residues in red wine using the QuEChERS extraction method (AOAC version), cleanup by dispersive solid-phase extraction (dSPE), followed by LC-MSMS analysis.
