HPLC-MS/MS separation for the tobacco alkaloids nicotine, nornicotine, cotinine, norcotinine, trans-3’-hydroxycotinine, and anabasine.
Forensic toxicologists face an ever-expanding list of compounds for analysis. This note presents work done using a next-generation triple quadrupole mass spectrometer with fast SRM acquisition speed for quantitation of 122 analytes in a single chromatographic run, where scan speed does not impact sensitivity or quantitative performance. Compounds analyzed include opiates, opioids, benzodiazepines, barbiturates, amphetamines, tricyclic antidepressants, illicit compounds, and more. For forensic use only.
There is growing environmental concern regarding the health impact of trace levels of pharmaceuticals and personal care products (PPCPs) in water resources. In response, the U.S. Environmental Protection Agency (EPA) published Method 1694, which determines dozens of PPCPs in water, soil, sediment, and biosolids by HPLC-MS/MS. We demonstrate a progressive approach to analyzing PPCPs in environmental sources of water at the ng/L level with online sample preparation using small volumes of water, thus saving time and reducing the cost of analysis.
A Thermo Scientific HPLC system is applied with the Thermo Scientific Syncronis aQ HPLC column for the analysis of alkaloids in less than three minutes with UV detection at 254 nm.
The Thermo Scientific HPLC system is applied for the analysis of nicotine, cotinine and trans-3-hydroxycotinine in urine. The samples were prepared using a SOLA SCX solid phase extraction (SPE) 96 well plate, and the separation performed in under 6 minutes on a Thermo Scientific Syncronis C18 HPLC column, with MS/MS detection. For Research Use Only. Not for use in diagnostic procedures.
