This application proof note demonstrates a determination of citrate and phosphate in pharmaceutical formulations using the method published in Thermo Fisher Scientific Application Note 164. In this proof note, the method is performed using Inuvion IC system.
Sodium benzoate and potassium benzoate are added to acidic foods as preservatives. Therefore, benzoate is regulated as a food additive. The determinations of benzoate in liquid food products was previously demonstrated in Application Note AN165. In this application proof note, the application is demonstrated on the same column, a Thermo Scientific™ Dionex™ IonPac™ AS18 (4 × 250 mm) anion-exchange column, using eluent generation and a suppressor upgraded to current technology. The application is facilitated by the Thermo Scientific™ Dionex™ Inuvion™ ion chromatography system.
This record presents a fast isocratic method to determine citric acid in carbonated beverages with a carbonate-based eluent and electrolytically regenerated suppression. This application can be run on any Thermo Scientific™ Dionex™ IC system equipped with suppressed conductivity detection. The carbonate eluent (see Method) can be generated electrolytically using Thermo Scientific™ Dionex™ RFIC™ technology.
This work uses IC with suppressed conductivity and mass spectrometry detection for organic acid determinations. This dual detection approach increases the information available from the sample. Samples were separated with a high-resolution Thermo Scientific™ Dionex™ IonPac™ AS11-HC-4μm column set using a Thermo Scientific™ Dionex™ Integrion™ HPIC™ system with suppressed conductivity detection, and an ISQ EC MS. Co-eluting organic acids can be accurately quantified with mass spectrometric detection, eliminating the need for the addition of methanol to the hydroxide eluent to enhance separation.
This application note updates previous Application Note 169. It presents a new method for the simultaneous determination of phosphate and citrate in carbonated soft drinks using the Dionex IonPac Fast Anion IIIA column. In addition, the sample injection was reduced from 1.2 to 0.4 μL to increase column life.
