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AU181: Rapid Screening of Sialic Acids in Glycoproteins by HPAE-PAD Be the first to rate this application

au181 rapid screening sialic acids glycoproteins by hpaepad

Description

Sialic acids are determined in five representative glycoproteins by acid hydrolysis followed by HPAE-PAD. Sialic acid determination by HPAE-PAD is specific and direct, eliminating the need for sample derivatization after hydrolysis. The rapid gradient method discussed separates Neu5Ac and Neu5Gc in <3 min with a total analysis time of 4.5 min. By using the Thermo Scientific Dionex CarboPac™ PA20 Fast Sialic Acid column, total analysis time is reduced, per sample eluent consumption and waste generation are reduced, and sample throughput is improved.
Market: BioPharma
Keywords: CarboPac, CHO cells, Glycoprotein, NANA, Neu5Ac, Neu5Gc, NGNA
Matrix: Glycoprotein
Uploaded on 9/29/2014.

For Research Use Only. Not for use in diagnostic procedures.