CAN123: Sugars in Honey Using HPAE-PAD: What Is the Best Column?

Instrument Type: IC

Honey is defined by the Codex Alimentarius as the natural, sweet substance produced by honey bees from the nectar of plants or from secretions of living parts of plants or excretions of plant sucking insects on the living parts of plants, which the bees collect, transform by combining with specific substances of their own, deposit, dehydrate, store and leave in the honeycomb to ripen and mature. High-performance anion-exchange chromatography with pulsed amperometric detection is commonly used to determine the carbohydrates in honey. This document evaluates CarboPac columns for that purpose.

TN125: Guidelines for Successful Use of Thermo Scientific Dionex AminoTrap Columns

Instrument Type: IC

Free amino acids and small peptides can interfere with HPAE-PAD monosaccharide analysis. An AminoTrap column in front of a CarboPac column can remove the aforementioned interference. This TN describes how to use the AminoTrap and how to test if it functioning correctly. The record reports an AminoTrap test method.

AU181: Rapid Screening of Sialic Acids in Glycoproteins by HPAE-PAD

Instrument Type: IC

Sialic acids are determined in five representative glycoproteins by acid hydrolysis followed by HPAE-PAD. Sialic acid determination by HPAE-PAD is specific and direct, eliminating the need for sample derivatization after hydrolysis. The rapid gradient method discussed separates Neu5Ac and Neu5Gc in <3 min with a total analysis time of 4.5 min. By using the Thermo Scientific Dionex CarboPac™ PA20 Fast Sialic Acid column, total analysis time is reduced, per sample eluent consumption and waste generation are reduced, and sample throughput is improved.