AU181: Rapid Screening of Sialic Acids in Glycoproteins by HPAE-PAD

Instrument Type: IC

Sialic acids are determined in five representative glycoproteins by acid hydrolysis followed by HPAE-PAD. Sialic acid determination by HPAE-PAD is specific and direct, eliminating the need for sample derivatization after hydrolysis. The rapid gradient method discussed separates Neu5Ac and Neu5Gc in <3 min with a total analysis time of 4.5 min. By using the Thermo Scientific Dionex CarboPac™ PA20 Fast Sialic Acid column, total analysis time is reduced, per sample eluent consumption and waste generation are reduced, and sample throughput is improved.