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AU181: Rapid Screening of Sialic Acids in Glycoproteins by HPAE-PAD
Description
Sialic acids are determined in five representative glycoproteins by acid hydrolysis followed by HPAE-PAD. Sialic acid determination by HPAE-PAD is specific and direct, eliminating the need for sample derivatization after hydrolysis. The rapid gradient method discussed separates Neu5Ac and Neu5Gc in <3 min with a total analysis time of 4.5 min. By using the Thermo Scientific Dionex CarboPac™ PA20 Fast Sialic Acid column, total analysis time is reduced, per sample eluent consumption and waste generation are reduced, and sample throughput is improved.| Market: | BioPharma |
| Keywords: | CarboPac, CHO cells, glycoprotein, NANA, Neu5Ac, Neu5Gc, NGNA, TN71, TN 71, TN 20, TN 70671, TN20, TN70671, AU 181 |
| Matrix: | Glycoprotein |
| Author: | Deanna Hurum and Jeffrey Rohrer |
| Affiliation: | Thermo Fisher Scientific, Sunnyvale, CA USA |
For Research Use Only. Not for use in diagnostic procedures.
