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TN186: The Effect of Working Electrode Gasket Thickness on the Sensitivity and Linearity of Carbohydrate Response by PAD with the Dionex™ CarboPac™ SA10 Column Be the first to rate this application


HPAE-PAD is widely used for determination of carbohydrates by measuring the electrical current generated by their oxidation at the surface of a gold electrode. Each amperometric detection cell includes an amperometric working electrode exposed to a sample flow-through channel, typically enclosed by a plastic gasket held in place by compression. Here, the sensitivity and linearity of six carbohydrates have been evaluated using 2, 15, and 62 mil gaskets under typical chromatographic conditions used for Thermo Scientific™ Dionex™ CarboPac™ SA10 column with and without postcolum addition.
Market: Food and Beverage
Keywords: fucose, xylose, glucose, Sucrose, PAD, fructose, glucosamine, HPAE-PAD, Thermo Scientific Dionex CarboPac PA20 column, Thermo Scientific Dionex CarboPac SA10 column
Matrix: <DI water>
Author: Beibei Huang; Jeffrey Rohrer
Affiliation: Thermo Fisher Scientific, Sunnyvale CA
Uploaded on 12/29/2016.