Ultrafast hydrophilic interaction UHPLC analysis of 2-AA labelled N-glycans from monoclonal antibodies (mAbs)
DescriptionThe Thermo Scientific Vanquish H system with fluorescence detection is applied for the rapid glycoprofiling of a commercial chimeric IgG1 mAb. The separation is performed on a Thermo Scientific Accucore-150-Amide HILIC column in under 2.5 minutes. Developed methodology offers an excellent analytical platform for ultrafast glycan profile comparison of various production batches.
|Keywords:||Biotherapeutics, Glycosylation, IgG, N-glycans, Rituximab, Monoclonal Antibodies, Glycoprofiling, NIBRT, Bioprocessing Research|
|Author:||Silvia Millán, Stefan Mittermayr, Amy Farrell and Jonathan Bones|
|Affiliation:||NIBRT, Dublin, Ireland|
|Status:||Proof of Concept|
For Research Use Only. Not for use in diagnostic procedures.