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Ultrafast hydrophilic interaction UHPLC analysis of 2-AA labelled N-glycans from monoclonal antibodies (mAbs) Be the first to rate this application


The Thermo Scientific Vanquish H system with fluorescence detection is applied for the rapid glycoprofiling of a commercial chimeric IgG1 mAb. The separation is performed on a Thermo Scientific Accucore-150-Amide HILIC column in under 2.5 minutes. Developed methodology offers an excellent analytical platform for ultrafast glycan profile comparison of various production batches.
Market: BioPharma
Keywords: Biotherapeutics, Glycosylation, IgG, N-glycans, Rituximab, Monoclonal Antibodies, Glycoprofiling, NIBRT, Bioprocessing Research
Matrix: Drug product
Author: Silvia Millán, Stefan Mittermayr, Amy Farrell and Jonathan Bones
Affiliation: NIBRT, Dublin, Ireland
Status: Proof of Concept
Uploaded on 12/20/2016.

For Research Use Only. Not for use in diagnostic procedures.