Rate
Ask the Expert
Suggest an Application
Add to Favorites
CreateParts List
Tell a Friend
PrintFind methods for your needs
In-depth protein glycosylation comparison of an innovator monoclonal antibody by hydrophilic interaction UHPLC
Description
The Thermo Scientific Vanquish H UHPC with Fluorescence detection is applied for the glycan profile comparison of trastuzumab innovator to a biosimilar candidate produced ‘in house’ using Chinese hamster ovary (CHO) cell line using HILIC-based chromatographic separation on Accucore 150-Amide-HILIC column. Developed methodology offers an excellent analytical platform for glycan profile comparison of multiples lots of both the innovator and biosimilar products| Market: | BioPharma |
| Keywords: | Glycosylation, IgG, N-glycans, Monoclonal Antibodies, Trastuzumab, Innovator, Biosimilars, NIBRT, Bioprocessing Research |
| Matrix: | Drug product |
| Author: | Silvia Millán, Anne Trappe, Amy Farrell and Jonathan Bones |
| Affiliation: | NIBRT, Dublin, Ireland |
| Status: | Proof of Concept |
For Research Use Only. Not for use in diagnostic procedures.
