In-depth protein glycosylation comparison of an innovator monoclonal antibody by hydrophilic interaction UHPLC
DescriptionThe Thermo Scientific Vanquish H UHPC with Fluorescence detection is applied for the glycan profile comparison of trastuzumab innovator to a biosimilar candidate produced ‘in house’ using Chinese hamster ovary (CHO) cell line using HILIC-based chromatographic separation on Accucore 150-Amide-HILIC column. Developed methodology offers an excellent analytical platform for glycan profile comparison of multiples lots of both the innovator and biosimilar products
|Keywords:||Glycosylation, IgG, N-glycans, Monoclonal Antibodies, Trastuzumab, Innovator, Biosimilars, NIBRT, Bioprocessing Research|
|Author:||Silvia Millán, Anne Trappe, Amy Farrell and Jonathan Bones|
|Affiliation:||NIBRT, Dublin, Ireland|
|Status:||Proof of Concept|
For Research Use Only. Not for use in diagnostic procedures.