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In-depth protein glycosylation comparison of an innovator monoclonal antibody by hydrophilic interaction UHPLC Be the first to rate this application


The Thermo Scientific Vanquish H UHPC with Fluorescence detection is applied for the glycan profile comparison of trastuzumab innovator to a biosimilar candidate produced ‘in house’ using Chinese hamster ovary (CHO) cell line using HILIC-based chromatographic separation on Accucore 150-Amide-HILIC column. Developed methodology offers an excellent analytical platform for glycan profile comparison of multiples lots of both the innovator and biosimilar products
Market: BioPharma
Keywords: Glycosylation, IgG, N-glycans, Monoclonal Antibodies, Trastuzumab, Innovator, Biosimilars, NIBRT, Bioprocessing Research
Matrix: Drug product
Author: Silvia Millán, Anne Trappe, Amy Farrell and Jonathan Bones
Affiliation: NIBRT, Dublin, Ireland
Status: Proof of Concept
Uploaded on 12/15/2016.

For Research Use Only. Not for use in diagnostic procedures.