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Displaying 1-3 of 3 results for Tag: biosimilars
In-depth protein glycosylation comparison of an innovator monoclonal antibody by hydrophilic interaction UHPLC
Instrument Type: UHPLCThe Thermo Scientific Vanquish H UHPC with Fluorescence detection is applied for the glycan profile comparison of trastuzumab innovator to a biosimilar candidate produced ‘in house’ using Chinese hamster ovary (CHO) cell line using HILIC-based chromatographic separation on Accucore 150-Amide-HILIC column. Developed methodology offers an excellent analytical platform for glycan profile comparison of multiples lots of both the innovator and biosimilar products
Reversed-Phase Separation of Intact Therapeutic Antibodies Using the Vanquish Flex UHPLC System
Instrument Type: HPLC (Biocompatible)Reversed-Phase chromatography (RP) of intact antibodies is one of the major analyses in biotherapeutics characterization. Here we show RP of several intact antibodies with The Thermo Scientific Vanquish Flex UHPLC in combination with the Thermo Scientific MAbPac-RP column. Column temperature was 80 °C; the mobile phase was pre-heated at the same temperature. Four antibodies were eluted with 10 minutes gradient. The elution of the intact antibodies resulted in very sharp and symmetrical peaks. Separation with the MAbPAc-RP were able to distinguish between a reference and a stressed antibody.
UHPLC Analysis of 2-Aminobenzamide-Labeled Glycans with the Vanquish Flex System
Instrument Type: HPLC (Biocompatible)HPLC of 2-aminobenzamide (2-AB) labeled glycans with fluorescence detection is one of the major analyses in biotherapeutics characterization. Here we show the separation of 2-AB-glycans originated by bovine fetuin and human IgG with The Thermo Scientific Vanquish Flex UHPLC. The Thermo Scientific GlycanPac AXR-1 column is used for the fetuin glycans. The Thermo Scientific Accucore 150-Amide-HILIC is used for the IgG glycans.
