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UHPLC Analysis of 2-Aminobenzamide-Labeled Glycans with the Vanquish Flex System
Description
HPLC of 2-aminobenzamide (2-AB) labeled glycans with fluorescence detection is one of the major analyses in biotherapeutics characterization. Here we show the separation of 2-AB-glycans originated by bovine fetuin and human IgG with The Thermo Scientific Vanquish Flex UHPLC. The Thermo Scientific GlycanPac AXR-1 column is used for the fetuin glycans. The Thermo Scientific Accucore 150-Amide-HILIC is used for the IgG glycans.| Market: | BioPharma |
| Keywords: | biosimilars, Biotherapeutics, Fluorescence detection, Bovine fetuin, glycan analysis, IgG |
| Matrix: | 82/18 (acetonitrile)/(ammonium formate 50 mM pH 4.2) (v/v) |
| Affiliation: | Thermo Fisher Scientific, Germering, Germany |
For Research Use Only. Not for use in diagnostic procedures.
