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AN1158: HPAE-PAD Determination of Carbohydrates in Honey to Evaluate Samples for Quality and Adulteration Be the first to rate this application


We developed a HPAE-PAD method to assay fructose, glucose, and to measure the entire profile of di- and trisaccharides in honey. The separation was achieved on the CarboPac™ PA210-4μm column. Carbohydrate detection was by PAD with a gold working electrode and therefore no sample derivatization was required. In this work, honey sugars of 12 honey samples have been characterized and quantitated by HPAE-PAD. In addition, HPAE-PAD profiling was demonstrated as a method to study the adulteration of honey samples with commercial sugar syrups. An ICS-6000 can be used for this application.
Market: Food and Beverage
Keywords: Carbohydrates, ICS-5000+, Honey, CarboPac PA210-4 μm column, food adulteration, sugar syrup, honey sugars authenticity, ICS-6000, TN 20, TN 70671, TN20, TN70671, AN 1158
Matrix: Honey
Author: Manali Aggrawal and Jeff Rohrer
Affiliation: Thermo Fisher Scientific, Sunnyvale, CA
Uploaded on 11/8/2016.

For Research Use Only. Not for use in diagnostic procedures.