AN1158: HPAE-PAD Determination of Carbohydrates in Honey to Evaluate Samples for Quality and Adulteration
DescriptionWe developed a HPAE-PAD method to assay fructose, glucose, and to measure the entire profile of di- and trisaccharides in honey. The separation was achieved on the CarboPac™ PA210-4μm column. Carbohydrate detection was by PAD with a gold working electrode and therefore no sample derivatization was required. In this work, honey sugars of 12 honey samples have been characterized and quantitated by HPAE-PAD. In addition, HPAE-PAD profiling was demonstrated as a method to study the adulteration of honey samples with commercial sugar syrups. An ICS-6000 can be used for this application.
|Market:||Food and Beverage|
|Keywords:||Carbohydrates, ICS-5000+, Honey, CarboPac PA210-4 μm column, food adulteration, sugar syrup, honey sugars authenticity, ICS-6000|
|Author:||Manali Aggrawal and Jeff Rohrer|
|Affiliation:||Thermo Fisher Scientific, Sunnyvale, CA|
For Research Use Only. Not for use in diagnostic procedures.