AN1158: HPAE-PAD Determination of Carbohydrates in Honey to Evaluate Samples for Quality and Adulteration

Instrument Type: IC

We developed a HPAE-PAD method to assay fructose, glucose, and to measure the entire profile of di- and trisaccharides in honey. The separation was achieved on the CarboPac™ PA210-4μm column. Carbohydrate detection was by PAD with a gold working electrode and therefore no sample derivatization was required. In this work, honey sugars of 12 honey samples have been characterized and quantitated by HPAE-PAD. In addition, HPAE-PAD profiling was demonstrated as a method to study the adulteration of honey samples with commercial sugar syrups. An ICS-6000 can be used for this application.

Dionex CarboPac PA210-Fast-4µm Column Performance Test Using its QAR Method

Instrument Type: IC

Before running any samples, Thermo Scientific recommends that you first confirm the performance of the column by reproducing the lot validation report chromatogram shipped with column. Compare your results with the one reported in the quality assurance report. At least three injections should be made. This record provides an eWorkflow for executing the QAR method on an ICS-5000+ system. An ICS-6000 can be used for this application.

AN1149: Profiling Fructosyloligosaccharides (FOS)-containing samples by HPAE-PAD

Instrument Type: IC

In this work, the oligo- and polysaccharide distribution of three different inulin samples has been characterized by HPAE-PAD. In addition, HPAE-PAD profiling was demonstrated as a method to determine FOS content in prebiotic samples and variation in prebiotics on addition of probiotics. We used Inulin-FOS and chicory root inulin as prebiotic samples. For probiotics, we used Yakult drink, a well known probiotic dairy product. In this method, separation of individual oligosaccharides in FOS was achieved on a Dionex CarboPac PA200 column. An ICS-6000 can be used for this application.

AN92: The Determination of Sugars in Molasses by High-Performance Anion Exchange with Pulsed Amperometric Detection

Instrument Type: IC

The accurate measurement of the amount of sugar in final molasses is economically important to sugar mills, because the purchase price of the final molasses is directly related to its sugar content. A high-performance anion exchange method with pulsed amperometric detection (HPAE-PAD) was developed to accurately determine the sugar concentrations of cane and beet final molasses samples.