AN1158: HPAE-PAD Determination of Carbohydrates in Honey to Evaluate Samples for Quality and Adulteration

Instrument Type: IC

We developed a HPAE-PAD method to assay fructose, glucose, and to measure the entire profile of di- and trisaccharides in honey. The separation was achieved on the CarboPac™ PA210-4μm column. Carbohydrate detection was by PAD with a gold working electrode and therefore no sample derivatization was required. In this work, honey sugars of 12 honey samples have been characterized and quantitated by HPAE-PAD. In addition, HPAE-PAD profiling was demonstrated as a method to study the adulteration of honey samples with commercial sugar syrups. An ICS-6000 can be used for this application.