Find methods for your needs

Determination of Trehalose and Protein by 2D-HPLC Be the first to rate this application


Many biopharmaceutical formulations use carbohydrates to enhance the stability of proteins. This method is an example of a "heart-cut" 2D HPLC method, separating the carbohydrate analyte from the protein. The method uses two charge aerosol detectors, as an example, but one can be substituted by UV. Calibration for the Trehalose is from 15.6 - 2000 ng o.c. If a second Corona Veo SD is used for the Protein, it uses the parameters described as "Veo SD".
Market: Other
Keywords: carbohydrate, Protein, BSA, Bovine serum albumin, trehalose
Matrix: Water
Author: Marc Plante
Affiliation: Thermo Fisher Scientific
Uploaded on 12/1/2015.

For Research Use Only. Not for use in diagnostic procedures.