Determination of Carbohydrates in Honey by HILIC-CAD

Instrument Type: HPLC-CAD

Honey is a complex, solution of saccharides with low water content resulting from the action of the enzyme invertase on sucrose, which creates other dissaccharides and polysaccharides. Other components include the enzymes themselves, organic acids, and minerals, all of which contribute to the flavors and preservative properties of this energy source. A UHPLC-CAD method uses HILIC to separate over 30 components of honey in 45 minutes.

Determination of Trehalose and Protein by 2D-HPLC

Instrument Type: HPLC-CAD

Many biopharmaceutical formulations use carbohydrates to enhance the stability of proteins. This method is an example of a "heart-cut" 2D HPLC method, separating the carbohydrate analyte from the protein. The method uses two charge aerosol detectors, as an example, but one can be substituted by UV. Calibration for the Trehalose is from 15.6 - 2000 ng o.c. If a second Corona Veo SD is used for the Protein, it uses the parameters described as "Veo SD".

Direct Determination of Unlabelled Carbohydrates, used in Biotech Formulations by HPLC-CAD

Instrument Type: HPLC-CAD

In many of the vaccines and other formulations that are used in the prevention and treatment of many conditions and illnesses contain carbohydrates, as a part of their formulation. Six carbohydrates were identified as important components in biotechnological formulations: sorbitol, mannitol, sucrose, lactose, maltose, and trehalose. These are separated by HILIC-HPLC and detected by the universal Corona charged aerosol detector.