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CAN119: Determination of Human Milk Oligosaccharides in Human Breast Milk by HPAE-PAD with On-Line Sample Cleanup
Description
In this study, a method for quantification of six human milk oligosaccharides, namely 2'-fucosyllactose, 3`-fucosyllactose, 3'-sialyllactose, 6'-sialyllactose, lacto-N-tetraose, and lacto-N-neotetraose in breast milk has been developed and validated. The method is based on the HPAE-PAD technique for carbohydrate determinations without sample derivatization. With an on-line sample cleanup using a polymeric reversed-phase column, a “dilute-and-shoot” sample preparation scheme was applied, which significantly reduces sample handling time and therefore increases sample throughput.| Market: | Food and Beverage |
| Keywords: | Dionex CarboPac PA1 Column, Electrochemical detection, PAD, HPAE-PAD, 2'-FL, 2'-fucosyllactose, 3-FL, 3-fucosyllactose, 3'-sialyllactose, 3'-SL, 6'-sialyllactose, 6'-SL, Dionex IonPac NG1 Column, Human Breast Milk, Human Milk Oligosaccharides, lacto-N-neotetraose, lacto-N-tetraose, LNnT, LNT, On-line Cleanup, TN21, TN71, TN 20, TN 70671, TN20, TN70671, CAN 119 |
| Matrix: | Human breast milk |
| Author: | Jing Tan, Felicia Chai, Chunyan Zhang |
| Affiliation: | Abbott Nutrition Research and Development, Singapore |
For Research Use Only. Not for use in diagnostic procedures.
