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AN105: Glycosylation Analysis of Human Serum Transferrin Glycoforms Using Pellicular Anion-Exchange Chromatography - HPAE-PAD Method
Description
Once the DNAPac PA100 fractions are obtained, PNGase F digestions can be performed. PNGase F, an amidase, removes N-linked oligosaccharides attached to glycoproteins. The released oligosaccharides from the different PNGase F digested fractions can be analyzed using HPAE-PAD (High-Performance Anion Exchange Chromatography with Pulsed Amperometric Detection) using a Dionex CarboPac PA100 column. Today we would start with a Dionex CarboPac PA200 column.| Market: | BioPharma |
| Keywords: | carbohydrate, Electrochemical detection, oligosaccharide, oligosaccharides, Human serum transferase, PAD, PNGase F, HPAE-PAD, TN21, TN71, TN110, TN 71, TN 20, TN 70671, TN20, TN70671, TN 21, AN 105, AN105 |
| Matrix: | Transferrin |
| Author: | Daniel Sui, John Statler, and Jeff Rohrer |
| Affiliation: | Thermo Fisher Scientific, Sunnyvale, CA USA |
For Research Use Only. Not for use in diagnostic procedures.
