Find methods for your needs
Refine by Feature
Displaying 1-2 of 2 results for Tag: transferrin
AN105: Glycosylation Analysis of Human Serum Transferrin Glycoforms Using Pellicular Anion-Exchange Chromatography - HPAE-PAD Method
Instrument Type: ICOnce the DNAPac PA100 fractions are obtained, PNGase F digestions can be performed. PNGase F, an amidase, removes N-linked oligosaccharides attached to glycoproteins. The released oligosaccharides from the different PNGase F digested fractions can be analyzed using HPAE-PAD (High-Performance Anion Exchange Chromatography with Pulsed Amperometric Detection) using a Dionex CarboPac PA100 column. Today we would start with a Dionex CarboPac PA200 column.
AN105: Glycosylation Analysis of Human Serum Transferrin Glycoforms Using Pellicular Anion-Exchange Chromatography
Instrument Type: ICVariation of glycoprotein sialylation has significant clinical implications. For example, human serum transferrin (HST) of cancer, rheumatoid arthritis, and haemochromatosis patients show an increased level of HST sialylation; whereas patients with heavy alcohol consumption show a high incidence of asialo glycoforms. Here we show separation of those glycoforms.