AN105: Glycosylation Analysis of Human Serum Transferrin Glycoforms Using Pellicular Anion-Exchange Chromatography - HPAE-PAD Method

Instrument Type: IC

Once the DNAPac PA100 fractions are obtained, PNGase F digestions can be performed. PNGase F, an amidase, removes N-linked oligosaccharides attached to glycoproteins. The released oligosaccharides from the different PNGase F digested fractions can be analyzed using HPAE-PAD (High-Performance Anion Exchange Chromatography with Pulsed Amperometric Detection) using a Dionex CarboPac PA100 column. Today we would start with a Dionex CarboPac PA200 column.