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Label-Free Determination by UHPLC with Charged Aerosol Detection of Glycans Separated by Size, Charge and Isomeric Structure
Description
This fast and sensitive method is suitable to directly measure the glycan content and profile of glycoproteins. Native N-linked glycans released from proteins by PNGase F are separated on the Thermo Scientific Vanquish UHPLC system by using the Thermo Scientific GlycanPac AXR-1 column. This column employs both weak anion exchange and reversed-phase separation mechanisms to separate glycans based on charge, isomerism and size. The native glycans are quantified directly by using charged aerosol detection.| Market: | BioPharma |
| Keywords: | glycan profiling, Universal Detection, mixed mode separation, label-free |
| Matrix: | Standard in deionized water |
| Author: | Dave Thomas |
| Affiliation: | Thermo Fisher Scientific |
For Research Use Only. Not for use in diagnostic procedures.
