Find methods for your needs

Refine by Feature

No filters available

Displaying 1-5 of 7 results for Tag: Universal Detection

Items per page:
Sort by

Accurately Monitor Protein PEGylation Reactions by HPLC-UV-CAD

Instrument Type: HPLC-CAD

This application provides a fast and accurate way for biopharmaceutical chemists to monitor PEGylation reactions by using HPLC with complementary UV/Vis and charged aerosol detection.Separation on the MAbPac RP resolves PEGylation reagents, unreacted IgG, and PEGylated IgG products that elute as a broad hump.

Profile Pyrethrins by UHPLC-CAD on Vanquish with Accucore Vanquish C18

Instrument Type: UHPLC

This application pairs a fast, high resolution separation on the Thermo Scientific Vanquish UHPLC system with high sensitivity detection by complimentary charged aerosol and diode array detectors to profile pyrethrins in plant extracts and related insecticidal formulations.

Determination of Steviosides by HPLC-CAD on UltiMate 3000

Instrument Type: HPLC-CAD

This application combines the Acclaim Trinity P1 with charged aerosol and diode array detection to quantify stevioside sweeteners used in foods and beverages. The two detectors are complimentary at higher concentrations of steviosides, but under the conditions used here the charged aerosol detector provides superior sensitivity for most of the analytes.

Determination of Hoodigosides by UHPLC-CAD on Vanquish

Instrument Type: Unspecified

This application highlights the complimentary sensitivity of UHPLC with charged aerosol and diode array detection for determination of hoodigosides in plant extracts and dietary supplements. Eight oxypregnane steroidal glycosides isolated from dried plant material are separated from one another and from excipients such as stearic acid within 20 min on a Thermo Scientific™ Vanquish ™ UHPLC system paired with a Thermo Scientific™ Vanquish™ C18 analytical column and detected by both charged aerosol and UV/Vis diode array detectors.

Detergent Profiling by HPLC-CAD

Instrument Type: UHPLC

The Thermo Scientific Acclaim Surfactant Plus column and charged aerosol detection are powerfully combined in the Thermo Scientific Vanquish UHPLC system to quickly characterize a complex mixture of chelants, ionic and non-ionic surfactants from a multipurpose alkaline cleaning concentrate. Because the charged aerosol detector can quantify any non-volatine analyte separated by HPLC, this method could be made quantitative for use in cleaning validation studies.