Label-Free Determination by UHPLC with Charged Aerosol Detection of Glycans Separated by Size, Charge and Isomeric Structure

Instrument Type: UHPLC

This fast and sensitive method is suitable to directly measure the glycan content and profile of glycoproteins. Native N-linked glycans released from proteins by PNGase F are separated on the Thermo Scientific Vanquish UHPLC system by using the Thermo Scientific GlycanPac AXR-1 column. This column employs both weak anion exchange and reversed-phase separation mechanisms to separate glycans based on charge, isomerism and size. The native glycans are quantified directly by using charged aerosol detection.