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AN160: Determination of Residual Trifluoroacetate in Protein Purification Buffers and Peptide Preparations by Reagent-Free Ion Chromatography
Description
In this note, we used RFIC to determine the concentration of residual trifluoroacetate (TFA) in samples of interest to the pharmaceutical industry. TFA is used with an acetonitrile gradient on a preparative RP- HPLC column to purify synthetic peptides. TFA is toxic, thus its removal must be reliably measured in products intended for clinical applications. High-capacity IonPac AS18 column was used to separate trace TFA from an excess of chloride, phosphate, and other anions in three different pharmaceutical buffers. This method improved sensitivity allowing more samples to be analyzed directly.| Market: | BioPharma |
| Keywords: | IonPac AS18, Peptide Preparations, Protein Purification Buffers, Reagent Free Ion Chromatography System, RFIC, RFIC-EG system, Suppressed conductivity, TFA, Trifluoroacetate |
| Matrix: | Protein purification buffers |
| Author: | Edward Kaiser and Jeffrey Rohrer |
| Affiliation: | Thermo Fisher Scientific |
For Research Use Only. Not for use in diagnostic procedures.
