AN107: Ions (Anions) in Physiological Fluids

Instrument Type: IC

One of the most important applications of IC in clinical chemistry is the analysis of physiological fluids such as urine, plasma, and serum both for inorganic and organic anions and cations. This application note outlines the analysis of physiological fluids using ion chromatography. Part one shows the anion method, the IonPac AS11 is coupled with the Anion Self-Regenerating Suppressor (ASRS) to dramatically reduce eluent conductivity, thus allowing the use of a rapidly increasing hydroxide gradient for the separation of an extensive group of monovalent through trivalent.

AN160: Determination of Residual Trifluoroacetate in Protein Purification Buffers and Peptide Preparations by Reagent-Free Ion Chromatography

Instrument Type: IC

In this note, we used RFIC to determine the concentration of residual trifluoroacetate (TFA) in samples of interest to the pharmaceutical industry. TFA is used with an acetonitrile gradient on a preparative RP- HPLC column to purify synthetic peptides. TFA is toxic, thus its removal must be reliably measured in products intended for clinical applications. High-capacity IonPac AS18 column was used to separate trace TFA from an excess of chloride, phosphate, and other anions in three different pharmaceutical buffers. This method improved sensitivity allowing more samples to be analyzed directly.