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SPE and LC-MS/MS method for the rapid determination of 25-Hydroxyvitamin D2 and 25-Hydroxyvitamin D3 from human plasma Be the first to rate this application

spe lcmsms method for rapid determination 25hydroxyvitamin d2 25hydroxyvitamin d3 from human plasma

Description

This method demonstrates a fast and efficient sample preparation using Thermo Scientific SOLA HRP for the determination of 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3 from human plasma. UHPLC tandem mass-spectrometry method, using a Thermo Scientific Syncronis C18 1.7 μm column provides a rapid separation of 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3 with a cycle time of 2 minutes while maintaining excellent peak shape. For Research Use Only. Not for use in diagnostic procedures.
Market: Pharma
Keywords: 25-hydroxyvitamin D2, 25-hydroxyvitamin D3, LC-MS/MS, SOLA HRP, Solid phase extraction, SPE, Syncronis C18
Matrix: Human plasma
Uploaded on 10/22/2014.

For Research Use Only. Not for use in diagnostic procedures.