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SPE and LC-MS/MS method for the rapid determination of 25-Hydroxyvitamin D2 and 25-Hydroxyvitamin D3 from human plasma

Description
This method demonstrates a fast and efficient sample preparation using Thermo Scientific SOLA HRP for the determination of 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3 from human plasma. UHPLC tandem mass-spectrometry method, using a Thermo Scientific Syncronis C18 1.7 μm column provides a rapid separation of 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3 with a cycle time of 2 minutes while maintaining excellent peak shape. For Research Use Only. Not for use in diagnostic procedures.Market: | Pharma |
Keywords: | 25-hydroxyvitamin D2, 25-hydroxyvitamin D3, LC-MS/MS, SOLA HRP, Solid phase extraction, SPE, Syncronis C18 |
Matrix: | Human plasma |
For Research Use Only. Not for use in diagnostic procedures.