Before running any samples, Thermo Scientific recommends that you first confirm the performance of the column by reproducing the lot validation report chromatogram shipped with column.Compare your results with the one reported in the quality assurance report. At least three injections should be made. This record provides an eWorkflow for executing the QAR method on an ICS-5000 system.<enter final 90 characters of description>

This application note describes the use of ion-exchange or ion-exclusion chromatography for the determination of five classes of compounds of interest to the brewing industry, including: carbohydrates, alcohols, organic acids, inorganic anions, and inorganic cations. One of two forms of electrochemical detection is used, pulsed amperometry or conductivity detection. This part of the application note describes organic acid analysis which uses conductivity detection. This was formerly Dionex AN46.

The Thermo Scientific HPLC system is applied for the analysis of glycolic and related acids. The separation is performed on a Thermo Scientific Acclaim Organic Acid (OA) column, with UV detection at 210 nm.

The Thermo Scientific HPLC system is applied for the comparison of the selectivity for sigma peptide standard with TFA vs formic acid. The separation is performed on a Thermo Scientific Acclaim 300 C18 HPLC column, with UV detection at 214 nm. Formic acid is a weaker acid, and a weaker ion-pairing agent, but gives a stronger signal for electrospray mass spectrometric detection. TFA is a better ion-pairing agent and yields better efficiency, but at the expense of MS performance.

This study shows a method for determining eight organic acids in wastewater using ion-exclusion chromatography and on-line carbonate removal. Normally, carbonate and inorganic anions are present in wastewater samples and interfere in organic acid analysis using ion exchange chromatography. In this study, the interferences are eliminated using ion-exclusion chromatography. Inorganic anions are eluted in the void volume and carbonate is removed by an on-line carbonate removal device.