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Comparison of the selectivity for sigma peptide standards on a Thermo Scientific Acclaim 300 C18 column with TFA vs formic acid
| # | Compound Name | Compound Class | RT (min) |
|---|---|---|---|
| 1 | Gly-Tyr | Peptide | 3.16 |
| 2 | Val-Tyr-Val | Peptide | 5.85 |
| 3 | Met-Enkephalin | Peptide | 8.14 |
| 4 | Angiotensin II | Peptide | 9.01 |
| 5 | Leu-Enkephalin | Peptide | 9.33 |
Description
The Thermo Scientific HPLC system is applied for the comparison of the selectivity for sigma peptide standard with TFA vs formic acid. The separation is performed on a Thermo Scientific Acclaim 300 C18 HPLC column, with UV detection at 214 nm. Formic acid is a weaker acid, and a weaker ion-pairing agent, but gives a stronger signal for electrospray mass spectrometric detection. TFA is a better ion-pairing agent and yields better efficiency, but at the expense of MS performance.| Market: | BioPharma |
| Keywords: | Acclaim 300 C18, Comparison, Formic acid, HPLC, Peptides, TFA, UV detection |
| Matrix: | N/A |
For Research Use Only. Not for use in diagnostic procedures.
