Rate
Ask the Expert
Suggest an Application
Add to Favorites
CreateParts List
Tell a Friend
PrintFind methods for your needs
Comparison of the selectivity for sigma peptide standards on a Thermo Scientific Acclaim 300 C18 column with TFA vs formic acid
Description
The Thermo Scientific HPLC system is applied for the comparison of the selectivity for sigma peptide standard with TFA vs formic acid. The separation is performed on a Thermo Scientific Acclaim 300 C18 HPLC column, with UV detection at 214 nm. Formic acid is a weaker acid, and a weaker ion-pairing agent, but gives a stronger signal for electrospray mass spectrometric detection. TFA is a better ion-pairing agent and yields better efficiency, but at the expense of MS performance.| Market: | BioPharma |
| Keywords: | Acclaim 300 C18, Comparison, Formic acid, HPLC, Peptides, TFA, UV detection |
| Matrix: | N/A |
For Research Use Only. Not for use in diagnostic procedures.
