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Comparison of the selectivity for sigma peptide standards on a Thermo Scientific Acclaim 300 C18 column with TFA vs formic acid Be the first to rate this application

comparison selectivity for sigma peptide standards on a thermo scientific acclaim 300 c18 column with tfa vs formic acid

Description

The Thermo Scientific HPLC system is applied for the comparison of the selectivity for sigma peptide standard with TFA vs formic acid. The separation is performed on a Thermo Scientific Acclaim 300 C18 HPLC column, with UV detection at 214 nm. Formic acid is a weaker acid, and a weaker ion-pairing agent, but gives a stronger signal for electrospray mass spectrometric detection. TFA is a better ion-pairing agent and yields better efficiency, but at the expense of MS performance.
Market: BioPharma
Keywords: Acclaim 300 C18, Comparison, Formic acid, HPLC, Peptides, TFA, UV detection
Matrix: N/A
Uploaded on 2/25/2015.

For Research Use Only. Not for use in diagnostic procedures.