A mix of five proteins in varying size was separated on a Thermo Scientific MabPac SEC-1 column using a Thermo Scientific Vanquish UHPLC system. The molecular weight range was 0.13 kDa – 670 kDa. Elution with high resolution of all five proteins was achieved within 15 minutes.

Size exclusion chromatography (SEC) is one of the major analyses in biotherapeutics characterization. Here we show a MAbPac SEC-1 column test with the Thermo Scientific Vanquish Flex UHPLC system.

Size exclusion chromatography (SEC) is one of the major analyses in biotherapeutics characterization. Here we show the separation of a mAb (monomer) and its aggregates (e.g., dimer) with the Thermo Scientific Vanquish Flex UHPLC system. Produced from biological cell cultures, mAbs may contain significant amounts of dimers, trimers and other higher order aggregates. The MAbPac SEC-1 is specially designed for analysis of mAbs and their aggregates.

Size-exclusion chromatography is used in carbohydrate research to determine molecular weight distributions of polysaccharides. Size-exclusion columns separate biological macromolecules by hydrodynamic size. Pulsed amperometric detection (PAD), provides sensitive and specific detection of carbohydrates. Here, fractionated pullulan standards were used to determine the separation range of linear carbohydrates.This application note shows how SEC can be coupled to PAD.

The Thermo Scientific LC system is applied for size exclusion chromatography analysis of four dextrans. The separation is performed on a Thermo Scientific Acclaim SEC-1000 HPLC column, with RI detection.