The Thermo Scientific Hypersil GOLD Silica column was used to separate seven steroids using a normal-phase HPLC method with UV detection at 254 nm.

The Thermo Scientific UltiMate 3000 RSLC system is applied for the analysis of strogens and progesterones. The separation is performed using a Thermo Scientific Acclaim Phenyl-1 column, with UV detection at 220 and 240 nm. for Research Use Only. Not for use in diagnostic procedures.

The separation of acidic and neutral drugs was successfully achieved on the Thermo Scientific Accucore C18 column, providing an excellent choice for the analysis of these drugs giving excellent retention time reproducibility and resolution.

The Thermo Scientific UltiMate 3000 system is applied for the determination of norethindrone and mestranol with progesterone as internal standard. The separation is performed on a Thermo Scientific Syncronis C8 HPLC column, with UV detection at 200 nm.

The rapid determination of progesterone is performed using a Thermo Scientific Accucore Phenyl-Hexyl HPLC column with a run time of less than four minutes.