There is increasing demand to provide rapid and selective screening techniques for a wide range of antibiotic drugs. Their abuse as a feed supplement in food animals is of growing concern and is subject to regulations globally. Creating screening methods for multiple analytes is more cost effective than dedicated methods for fewer analytes. This application shows the advantages of using the Thermo Scientific Accucore Vanquish C18 UHPLC 1.5 µm column and Vanquish UHPLC system for the analysis of thirty-six antibiotics.

There is growing environmental concern regarding the health impact of trace levels of pharmaceuticals and personal care products (PPCPs) in water resources. In response, the U.S. Environmental Protection Agency (EPA) published Method 1694, which determines dozens of PPCPs in water, soil, sediment, and biosolids by HPLC-MS/MS. We demonstrate a progressive approach to analyzing PPCPs in environmental sources of water at the ng/L level with online sample preparation using small volumes of water, thus saving time and reducing the cost of analysis.

The analysis was performed on a Thermo Scientific Dionex UltiMate 3000 HPLC system and a Thermo Scientific Syncronis aQ HPLC column. All USP criteria was exceeded. Resolution between 2-phenylacetamide and penicillin G potassium was 6.1 (USP criteria of not less than 2.0). Tailing factor for penicillin G potassium was 1.5 (USP criteria not more than 2.0). The % RSD for replicate injections of standard solution was 0.2% (USP criteria not more than 2.0%). The relative retention time of 2 phenylacetamide peak with respect to penicillin G potassium peak was 0.7 (USP criteria about 0.8).

The Thermo Scientific HPLC system is applied for the analysis of penicillin V. The separation is performed on a Thermo Scientific Hypersil GOLD CN HPLC column, with UV detection at 220 nm.

The Thermo Scientific HPLC system is applied for the analysis of penicillins. The separation is performed on a Thermo Scientific Hypersil GOLD CN HPLC column, with UV detection at 220 nm.