The Thermo Scientific Dionex UltiMate 3000 LC system is applied to the separation of related impurities and Intermediate stage separation of Guanine. The separation was performed on a Thermo Scientific Acclaim Mixed Mode WCX-1 column with a UV detection at 254 nm. With a run time of 15 minutes, the method allows easy separation of intermediate stages and related impurities of Guanine.

The Thermo Scientific HPLC system is applied for the analysis of basic drugs. The separation is performed on a Thermo Scientific Acclaim 120 C18 column, with UV detection at 220 nm. This application is used as a routine lot qualification test for the Acclaim 120 C18 column stationary phase. Low silanol activity preserves the good peak shape for amitriptyline as the sample concentration decreases (amitriptyline mass for trace A is 1200 ng, for trace B is 400 ng, and for trace C 94 ng).

The Thermo Scientific HPLC system is applied for the determination of benzodiazepines. The separation is performed on a Thermo Scientific Hypersil GOLD Phenyl 1.9 µm UHPLC column, with UV detection at 254 nm.

The Thermo Scientific HPLC system is applied for the analysis of a phenone test mix. The separation is performed on a Thermo Scientific Hypersil GOLD HPLC column, with UV detection at 254 nm.

The Thermo Scientific HPLC system is applied for the analysis of vitamin C. The separation is performed on a Thermo Scientific Hypersil GOLD PFP HPLC column, with UV detection at 254 nm.