A Thermo Scientific HPLC system is applied with the Thermo Scientific Syncronis aQ HPLC column for the analysis of alkaloids in less than three minutes with UV detection at 254 nm.

The Thermo Scientific Acclaim Trinity P1 column was used to separate nicotine and its counterion. The nicotine and salicylate were separated using a phosphate buffer system to permit isocratic elution and UV detection at 210 nm. Alternative wavelengths are 258 nm for nicotine and 296 nm for salicylate.

The Thermo Scientific Acclaim Trinity P1 column was used to separate nicotine and its counterion. The nicotine and tartrate were separated using a phosphate buffer system to permit isocratic elution and UV detection at 210 nm.

The Thermo Scientific HPLC system is applied for the analysis of nicotine, cotinine and trans-3-hydroxycotinine in urine. The samples were prepared using a SOLA SCX solid phase extraction (SPE) 96 well plate, and the separation performed in under 6 minutes on a Thermo Scientific Syncronis C18 HPLC column, with MS/MS detection. For Research Use Only. Not for use in diagnostic procedures.

The Thermo Scientific HPLC system is applied for the analysis of nicotine. The separation is performed on a Thermo Scientific Hypersil GOLD HPLC column, with UV detection at 254 nm.