Determination of Isohumulones in Beer using Rapid Separation On-Line SPE

Instrument Type: UHPLC

The BeerNHop solution for high throughput was applied to analyze untreated beer. Automated analyte enrichment was done by on-line solid phase extraction (SPE) on a Thermo Scientific Hypersil GOLD C8 column. Then, the separation was performed on a Hypersil GOLD 1.9 µm column with UV detection at 270 nm. With a total run time of 9 minutes, the method allows very fast screening for isohumulones in beer. Hence, beer bitterness is quantified and the isohumulone pattern is identified in a matter of minutes. The BeerNHop solution is a valuable tool for the daily business of each brewery.

Determination of Isohumulones using Standard On-Line SPE

Instrument Type: HPLC

The BeerNHop solution using an on-line SPE-HPLC method was applied for the analysis of the Thermo Scientific isohumulones standard. Automated analyte enrichment was done by solid phase extraction (SPE) on a Thermo Scientific Hypersil GOLD C8 column. Then, the separation was performed on a Hypersil GOLD 3 μm column with UV detection at 270 nm. With a total run time of 15 minutes, the method allows fast screening for isohumulones in beer. Hence, beer bitterness is quantified and the isohumulone pattern is identified within minutes.

Determination of Isohumulones in Beer

Instrument Type: HPLC

Untreated beer was directly injected onto a Thermo Scientific Hypersil GOLD 3 μm column. With a total run time of 15 minutes, the method allows fast screening for isohumulones in beer. Hence, beer bitterness is quantified and the isohumulone pattern is identified within minutes. The BeerNHop solution is a valuable tool for the daily business of each brewery.